{"id":11809,"date":"2020-05-05T23:15:46","date_gmt":"2020-05-05T20:15:46","guid":{"rendered":"https:\/\/www.synevo.ro\/shop\/borrelia-burgdorferi-adn-din-capusa\/"},"modified":"2025-05-12T12:57:59","modified_gmt":"2025-05-12T09:57:59","slug":"borrelia-burgdorferi-adn-din-capusa","status":"publish","type":"product","link":"https:\/\/old.synevo.md\/ru\/shop\/borrelia-burgdorferi-adn-din-capusa\/","title":{"rendered":"Borrelia burgdorferi ADN din capu\u0219\u0103"},"content":{"rendered":"
\n

\u041e\u0431\u0449\u0430\u044f \u0438\u043d\u0444\u043e\u0440\u043c\u0430\u0446\u0438\u044f <\/em><\/strong><\/p>\n

Boala Lyme\u00a0este o\u00a0afectiune multisistemica aparuta dupa muscatura de capusa infectata cu Borrelia burgdorferi. Studiul diferentelor antigenice dintre tulpinile izolate in America si Europa a permis recunoasterea implicarii in patologia umana a trei genospecii care alcatuiesc complexul Borrelia burgdorferi sensu lato: Borrelia burgdorferi sensu stricto<\/em> (ce cuprinde toate tulpinile izolate in America de Nord), B. garinii<\/em> si B. afzelii<\/em>. In Europa circula toate cele trei genospecii, dar predomina B. garinii si B. afzelii1;2;3;6;7<\/sup><\/p>\n

Tehnicile moleculare reprezinta o alternativa in diagnosticul bolii Lyme\u00a0 si se bazeaza pe amplificarea unei regiuni specifice a genomului bacterian. Acestea au aplicabilitate pentru toate \u00a0produsele \u00a0patologice recoltate in diferite stadii ale infectiei, dar se recomanda a fi utilizate doar la pacientii care au un rezultat pozitiv sau echivoc la testele serologice6<\/sup>.<\/p>\n

Recomandari pentru determinarea Borrelia \u2013 ADN <\/em><\/strong>\u2013 diagnosticul bolii Lyme; astfel1<\/sup>:<\/p>\n

Stadiul I \u2013 biopsie cutanata din eritemul migrator;<\/p>\n

Stadiul II \u2013 biopsie cutanata din eritemul migrator diseminat sau LCR in caz de neuroborelioza;<\/p>\n

Stadiul III \u2013 biopsie cutanata in acromodermatita cronica atrofica, lichid sinovial in artrita Lyme sau LCR in neuroborelioza.<\/p>\n

Tehnica PCR are \u00a0o sensibilitate mai mare decat cultura in cazul prelevatelor bioptice, a sangelui si a lichidului articular, spre deosebire de LCR si urina unde rezultatele\u00a0 nu au fost satisfacatoare 1;7<\/sup>.<\/p>\n

Studii efectuate prin analiza PCR a lichidului articular provenit de la pacienti cu artrita Lyme au demonstrat o sensibilitate si specificitate excelenta (Nocton, 1994; Dumler, 2001). Spre deosebire de acestea, studiile efectuate pe LCR provenit de la pacienti cu neuroborelioza acuta sau cronica au demonstrat o imbunatatire minima a sensibilitatii, in comparatie cu cultura.<\/p>\n

Pregatire pacient<\/em><\/strong> \u2013 nu este necesara4<\/sup>.<\/p>\n

Specimen recoltat<\/em><\/strong> \u2013 a) biopsie cutanata (fragment in tampon fosfat salin); b) lichid cefalorahidian recoltat prin punctie lombara; c) lichid sinovial; d) sange venos; e) capusa extrasa de la locul intepaturii4<\/sup>.<\/p>\n

Recipient de recoltare<\/em><\/strong>\u00a0\u2013 vacutainer ce contine EDTA ca anticoagulant pentru proba de sange venos si recipient steril pentru celelalte produse biologice4<\/sup>.<\/p>\n

Cantitate recoltata <\/em><\/strong>\u2013 b) 2 mL; c) 2 mL; d) 3 mL.<\/p>\n

Prelucrare necesara dupa recoltare <\/em><\/strong>\u2013 probele nu<\/strong> vor fi centrifugate4<\/sup>.<\/p>\n

Cauze de respingere a probei<\/em><\/strong> \u2013 cantitate insuficienta; probe vechi, coagulate, hemolizate; folosirea heparinei ca anticoagulant4<\/sup>.<\/p>\n

Stabilitate proba <\/em><\/strong>\u2013 probele de sange, produs din leziune, LCR, lichid articular sunt stabile 3 zile\u00a0<\/em>la 2-4\u00b0C4<\/sup>.<\/p>\n

Metoda<\/em><\/strong> \u2013 Real-time PCR Light-Cycler: reactie de polimerizare in lant cu detectie in timp real a produsului PCR\u00a0 acumulat, prin masurarea fluorescentei emise (test calitativ)4<\/sup>.<\/p>\n

Valori de referinta <\/em><\/strong><\/p>\n

Borrelia \u2013 ADN: nedetectabil4<\/sup>.<\/p>\n

Limita de detectie<\/em><\/strong> \u2013 276 copii\/ mL4<\/sup>.<\/p>\n

Interpretarea rezultatelor<\/em><\/strong><\/p>\n

Un rezultat pozitiv indica prezenta de ADN Borrelia in proba recoltata6<\/sup>.<\/p>\n

Limite si interferente<\/em><\/strong><\/p>\n

Un rezultat negativ nu exclude infectia cu Borrelia, in timp ce un rezultat pozitiv in afara contextului clinic, nu confirma diagnosticul 3;6;7<\/sup>.<\/p>\n

Testul PCR trebuie folosit ca o metoda care imbogateste paleta de investigatii in boala Lyme si nu poate inlocui celelalte metode. Rezultatele obtinute vor fi corelate obligatoriu cu serologia, datele clinice si epidemiologice ale pacientilor6<\/sup>.<\/p>\n

\u0411\u0438\u0431\u043b\u0438\u043e\u0433\u0440\u0430\u0444\u0438\u044f<\/p>\n

1. Bettina Wilske, Barbara J. B. Johnson, and Martin E. Schriefer. Borrelia In:Patrick R. Murray et al. \u2013 \u201cManual of Clinical Microbiology, 9th ed. , 2007 , 62: 971- 986.<\/p>\n

2. Betty A. Forbes, Daniel S. Sahm, Alice S. Weissfeld. The Spirochetes.In Diagnostic Microbiology, Twelfth Edition, 2007, 48: 533\u2013541.<\/p>\n

3. Koneman\u2019s, Spirochetal infections. In Color Atlas and Textbook of Diagnostic Microbiology, Sixth Edition, 2006, 20: 1125-1150.<\/p>\n

4. Laborator Synevo. Referintele specifice tehnologiei de lucru utilizate 2015. Ref Type: Catalog.<\/p>\n

5. Mayo Clinic. Mayo Medical Laboratories. Test Catalog: Lyme Disease (Borrelia burgdorferi), Molecular Detection, PCR. www.mayomedicallaboratories.com. Ref Type: Internet Communication.<\/p>\n

6. Volker von Baehr, Christa Liebenthal, Brita Gaida, Frank- Peter Schmidt, Rudiger von Baehr und Hans-Dieter Volk. Evaluation of the diagnostic significance of the lymphocyte proliferation test in patients with Lyme borreliosis. In J Lab Med, 2007; 31(3).<\/p>\n

7. P. Rocco La Sala, Michael B. Smith. Spirochete Infections. In Henry\u2019s Clinical Diagnosis and Management by Laboratory Methods, Twenty-First Edition, 2007, 58, 1059- 1074.<\/p>\n<\/div>","protected":false},"excerpt":{"rendered":"

Informatii generale Boala Lyme\u00a0este o\u00a0afectiune multisistemica aparuta dupa muscatura de capusa infectata cu Borrelia burgdorferi. Studiul diferentelor antigenice dintre tulpinile izolate in America si Europa a permis recunoasterea implicarii in patologia umana a trei genospecii care alcatuiesc complexul Borrelia burgdorferi sensu lato: Borrelia burgdorferi sensu stricto (ce cuprinde toate tulpinile izolate in America de Nord), … Continued<\/a><\/p>","protected":false},"featured_media":0,"comment_status":"closed","ping_status":"closed","template":"","meta":[],"product_brand":[],"product_cat":[968,971],"product_tag":[],"class_list":{"0":"post-11809","1":"product","2":"type-product","3":"status-publish","5":"product_cat-teste-de-biologie-moleculara","6":"product_cat-teste-moleculare-pentru-diagnosticul-infectiilor","8":"first","9":"instock","10":"sold-individually","11":"shipping-taxable","12":"purchasable","13":"product-type-simple"},"_links":{"self":[{"href":"https:\/\/old.synevo.md\/ru\/wp-json\/wp\/v2\/product\/11809","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/old.synevo.md\/ru\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/old.synevo.md\/ru\/wp-json\/wp\/v2\/types\/product"}],"replies":[{"embeddable":true,"href":"https:\/\/old.synevo.md\/ru\/wp-json\/wp\/v2\/comments?post=11809"}],"wp:attachment":[{"href":"https:\/\/old.synevo.md\/ru\/wp-json\/wp\/v2\/media?parent=11809"}],"wp:term":[{"taxonomy":"product_brand","embeddable":true,"href":"https:\/\/old.synevo.md\/ru\/wp-json\/wp\/v2\/product_brand?post=11809"},{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/old.synevo.md\/ru\/wp-json\/wp\/v2\/product_cat?post=11809"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/old.synevo.md\/ru\/wp-json\/wp\/v2\/product_tag?post=11809"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}